Typing of healthcare associated MRSA from Australia in the 1970s runescape 07 gold revealed a novel clone, ST2249 MRSA III (CC45), present from 1973 to 1979. This clone was present prior to the Australian epidemic caused by the recombinant clone, ST239 MR... másTyping of healthcare associated MRSA from Australia in the 1970s runescape 07 gold revealed a novel clone, ST2249 MRSA III (CC45), present from 1973 to 1979. This clone was present prior to the Australian epidemic caused by the recombinant clone, ST239 MRSA III. This study aimed to characterise the genome of ST2249 MRSA III in order to establish its relationship to other MRSA clones. DNA microarray analysis was conducted and a draft genome sequence of ST2249 was obtained. The recombinant structure of the ST2249 genome was revealed by comparisons to publicly available ST239 and ST45 genomes. Microarray analysis of genomic DNA of 13 ST2249 isolates showed gross similarities with the ST239 chromosome in a segment around the origin of replication and with ST45 for the remainder of the chromosome. Recombination breakpoints were precisely determined by the changing pattern of nucleotide polymorphisms in the genome sequence of ST 2249 isolate SK1585 compared with ST239 and ST45. One breakpoint was identified to the rig... menos
